多肽修饰:
C-端标记技术:
o C端酰胺化(Amidation,C-termainal) o 醛基化(Aldehydes) o 醇基化(Alcohols) o pNA (p-Nitroanilide) o AMC o AFC o 巯基乙胺化(Cysteamide) o 酯基化(Ester) o ØN-烷基化(N-Alkyl Amides)
N-端标记技术:
o 乙酰化Acetylated (Acetylated) o Palmytolylo HYNIC o 生物素标记(Biotinylated) o Br乙酰化(Bromoacetylated) o 螯合反应(DOTA,DTPA conjugated) o 甲醛化(Formylated) o 十四烷基,十八烷基化(Myristoylated) o 琥珀酰化、棕榈酸化、苹果酸化、脂肪酸化等(Succinylated)
荧光标记修饰:
o C端修饰:AFC, AMC, Dap(Dnp), Lys(Dye), pNA, Rh110²
o N端修饰:Bodipy-FL, Cy3, Cy5, Texas Red, 5-Tamra, 5-lodoacetamido fluorescein
Rhodamine 110 and² Rhodamine B Luciferin, EDANS
FAM, FITC, MCA, Rox, Sulforhodamine 101,² Ø5-TAMRA
环化反应:
o 首尾成环,中间成环,特殊成环(N -> C or Head to Tail) o 2对二硫键,3对二硫键(Disulfide (S-S bond formation) .Trisulfide formation) o 天然产物活性肽成环(Cyclic-natural peptides
甲基化:
o 侧链甲基化Lys(For),Lys(Me), Lys(Me)2, Lys(Me)3, Arg(Me)2 symmetrical, D-Tyr(Me),D-Tyr(Et) o N端甲基化(N-Me-Arg,N-Me-Asp, N-Me-Glu, N-Me-Leu, N-Me-Nle, N-Me-Nva, N-Me-Phe, N-Me-S N-Me-Ser, N-Me-Trp, N-Me-Thr, N-Me-Val)
几种常见的特殊修饰:
o 各种偶联(BSA, KLH conjugated peptides for antibody production ) o 磷酸化(Phosphoserine, Phosphothreonine, Phosphotyrosine) o 磺化(Sulfated Tyrosine or Serine)o MAPS (Multiple Antigenic Peptide) o 糖肽(Glycopeptides) o PEG修饰(PEGylation)
o C端酰胺化(Amidation,C-termainal) o 醛基化(Aldehydes) o 醇基化(Alcohols) o pNA (p-Nitroanilide) o AMC o AFC o 巯基乙胺化(Cysteamide) o 酯基化(Ester) o ØN-烷基化(N-Alkyl Amides)
N-端标记技术:
o 乙酰化Acetylated (Acetylated) o Palmytolylo HYNIC o 生物素标记(Biotinylated) o Br乙酰化(Bromoacetylated) o 螯合反应(DOTA,DTPA conjugated) o 甲醛化(Formylated) o 十四烷基,十八烷基化(Myristoylated) o 琥珀酰化、棕榈酸化、苹果酸化、脂肪酸化等(Succinylated)
荧光标记修饰:
o C端修饰:AFC, AMC, Dap(Dnp), Lys(Dye), pNA, Rh110²
o N端修饰:Bodipy-FL, Cy3, Cy5, Texas Red, 5-Tamra, 5-lodoacetamido fluorescein
Rhodamine 110 and² Rhodamine B Luciferin, EDANS
FAM, FITC, MCA, Rox, Sulforhodamine 101,² Ø5-TAMRA
环化反应:
o 首尾成环,中间成环,特殊成环(N -> C or Head to Tail) o 2对二硫键,3对二硫键(Disulfide (S-S bond formation) .Trisulfide formation) o 天然产物活性肽成环(Cyclic-natural peptides
甲基化:
o 侧链甲基化Lys(For),Lys(Me), Lys(Me)2, Lys(Me)3, Arg(Me)2 symmetrical, D-Tyr(Me),D-Tyr(Et) o N端甲基化(N-Me-Arg,N-Me-Asp, N-Me-Glu, N-Me-Leu, N-Me-Nle, N-Me-Nva, N-Me-Phe, N-Me-S N-Me-Ser, N-Me-Trp, N-Me-Thr, N-Me-Val)
几种常见的特殊修饰:
o 各种偶联(BSA, KLH conjugated peptides for antibody production ) o 磷酸化(Phosphoserine, Phosphothreonine, Phosphotyrosine) o 磺化(Sulfated Tyrosine or Serine)o MAPS (Multiple Antigenic Peptide) o 糖肽(Glycopeptides) o PEG修饰(PEGylation)